16bc16ef-588a-47e2-803e-03b4acb85dca Ramond Pierre IFREMER 02 98 22 42 60 Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Pierre.Ramond@ifremer.fr author Raffaele Siano IFREMER raffaele.siano@ifremer.fr pointOfContact Sourisseau Marc IFREMER marc.sourisseau@ifremer.fr author 2025-12-13T15:59:45.324967Z ISO 19115-3 - SEXTANT 1.0 WGS 84 (EPSG:4326) EPSG 8.6 Metabarcoding of Coastal Ecosystems 2016-04-18T00:00:00 creation FR-330-715-368-00032-DATARMOR_BIOINFO_POHEM DOI:10.12770/16bc16ef-588a-47e2-803e-03b4acb85dca A total number of 277 water samples were collected during distinct oceanographic cruises or at fixed stations across coastal systems of France and Senegal. The seawater samples were progressively filtered onto size-fractionated filters (representing micro, nano and pico-plankton). Metabarcoding of the V4 domain of the Eukaryotic 18S rDNA region was carried out to characterize the genetic diversity of the sampled communities. Genomic DNA was extracted following the DNA extraction kit Nucleospin Plant II (Macherey-Nagel) and the V4 markers were amplified with a taq polymerase (Phusion High-Fidelity PCR Master Mix with GC Buffer). Sequencing was performed by the Genotoul sequencing platform (get.genotoul.fr) with the Illumina MISeq method (2x250 bp). The present dataset gathers the different results issued from sequencing. This dataset was submitted to sequence cleaning, filtering, taxonomic assignment and OTU clustering, which resulted in a final dataset also presented. Projet POHEM - Laboratoire Dyneco PELAGOS and Station Biologique de Roscoff Ramond Pierre IFREMER 02 98 22 42 60 Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Pierre.Ramond@ifremer.fr author Siano Raffaele IFREMER 02 98 22 42 04 02 98 22 45 48 Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Raffaele.Siano@ifremer.fr author Sourisseau Marc IFREMER 02 98 22 43 61 02 98 22 45 48 Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Marc.Sourisseau@ifremer.fr author IFREMER - SISMER sismer@ifremer.fr publisher https://sextant.ifremer.fr/geonetwork/srv/api/records/16bc16ef-588a-47e2-803e-03b4acb85dca/attachments/Sans%20titre.png /Biological Environment/Bioinformatics Thèmes Sextant 2025-12-09 publication geonetwork.thesaurus.local.theme.sextant-theme Metabarcoding Protist Coastal Ecosystem Variables ODATIS 2023-10-13 publication geonetwork.thesaurus.local.theme.odatis_variables Bioinformatics ODATIS aggregation parameters and Essential Variable names 2025-07-29 publication geonetwork.thesaurus.external.theme.NVS.OD1 Analysis data Type de jeux de donnée ODATIS 2025-12-09 publication geonetwork.thesaurus.local.theme.type_jeux_donnee Data under Licence ouverte / Open licence : http://www.etalab.gouv.fr/pages/licence-ouverte-open-licence-5899923.html grid utf8 oceans -22.015156 0.01 8.700516 50.8460719 2009-01-07 2015-11-25 https://doi.org/10.12770/16bc16ef-588a-47e2-803e-03b4acb85dca DOI DOI of the dataset DOI of the dataset https://data-dataref.ifremer.fr/bioinfo/ifremer/dyneco/POHEM/ WWW:DOWNLOAD Download link Download link /home/ref-bioinfo-public/ifremer-controlled/dyneco/POHEM NETWORK:LINK A link to the dataset can be sent upon request. The link will access a two folder repository : - [data] contains: [dna-sequence-raw] inculding raw sequencing data of each sample for the reverse and forward V4 markers (R1 & R2); [otu-table] which is our final table (after sequence cleaning, filtering, taxonomic assignment with PR² (Guillou et al., 2013) and OTU clustering with swarm2 (Mahé et al., 2015). - [operation] contains diverse operations carried out by the sequencing platform (see inside) and a merging of reverse and forward sequences [sequence-merging]