173c0414-a3ca-4a79-b6b2-cd424ee90593 Arnaud Lagorce IFREMER, Interaction Hôtes-Pathogènes-Environnement (IHPE) 06 61 62 01 08 Université de Montpellier - Campus Triolet - Place E. Bataillon Montpellier France arnaud.lagorce@ifremer.fr pointOfContact 2025-12-13T11:46:33.807213Z ISO 19115-3 - SEXTANT 1.0 0 WGS 84 (EPSG:4326) EPSG 8.6 DNA marker sequences of vibrio bacteria sampled from oysters and seawater in Thau lagoon (2015, France) 2021-02-13 creation 2022-06-13 publication FR-330-715-368-00032-IFR_BIOINFO_SEQ_VIBRIO_OYSTERS_THAU DOI:10.12770/173c0414-a3ca-4a79-b6b2-cd424ee90593 Vibrio bacteria sampled from juvenile oysters and seawater collected in Thau Lagoon (Languedoc-Roussillon, France) in October 2015 during a mortality event were genotyped using hsp60, rctB, topA and mreB protein-coding genes IFREMER, Interaction Hôtes-Pathogènes-Environnement (IHPE) Arnaud Lagorce IFREMER, Interaction Hôtes-Pathogènes-Environnement (IHPE) 06 61 62 01 08 Université de Montpellier - Campus Triolet - Place E. Bataillon Montpellier France arnaud.lagorce@ifremer.fr author National Centre for the Development of the Oceans, Brittany Oceanological Centre Technopole de Brest-Iroise Brest 29280 France sismer@ifremer.fr web site publisher Haffner Philippe IFREMER author Destoumieux-Garzon Delphine IFREMER delphine.destoumieux.garzon@ifremer.fr author https://sextant.ifremer.fr/geonetwork/srv/api/records/173c0414-a3ca-4a79-b6b2-cd424ee90593/attachments/BioinfoMarine.png Habitats and biotopes GEMET - INSPIRE themes, version 1.0 2018-07-27T14:39:48 publication 2018-07-27 publication geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme /Biological Environment/Bioinformatics Thèmes Sextant 2025-12-09 publication geonetwork.thesaurus.local.theme.sextant-theme Variables ODATIS 2023-10-13 publication geonetwork.thesaurus.local.theme.odatis_variables Bioinformatics ODATIS aggregation parameters and Essential Variable names 2025-07-29 publication geonetwork.thesaurus.external.theme.NVS.OD1 Analysis data Type de jeux de donnée ODATIS 2025-12-09 publication geonetwork.thesaurus.local.theme.type_jeux_donnee CC-BY (Creative Commons - Attribution) grid utf8 -6.00 11.932 40.9268 51.20 /home/ref-bioinfo-public/ifremer/ihpe/2015_Vibrio_Thau-Lagoon_genotyping NETWORK:LINK https://data-dataref.ifremer.fr/bioinfo/ifremer/ihpe/2015_Vibrio_Thau-Lagoon_genotyping/ WWW:DOWNLOAD Lien de téléchargement Lien de téléchargement https://doi.org/10.12770/173c0414-a3ca-4a79-b6b2-cd424ee90593 DOI Digital Object Identifier (DOI) Oyster and seawater sampling: Juvenile oysters (Pathogen free animals produced in hatchery) and seawater were sampled in Thau Lagoon (Languedoc-Roussillon, France) in October 2015 during a mortality event. Seawater wassize-fractionated by sequential filtration as described by Bruto et al., 2016.Bacterial sampling: Oysters and large seawater particles fractions (> 60μm) were ground (Ultra-Turrax, IKA) and 100μL were plated on Vibrio selective media (thiosulfate-citrate-bile salts sucrose agar, TCBS). The others filters (5, 1 and 0.22 μm) were directly placed on TCBS agar plate and incubated 2 days at 20°C. Around 100 colonies for each sample were randomly picked, re-steaked first on TCBS and then on Zobell agar (4g.L-1bactopeptone, 1 g.L-1yeast extract and 15g.L-1agar in sterile seawater, pH 7.4) and stock cultures were stored at −80 °C in Zobell containing 15% glycerol (v/v). Population structure analysis: Isolates were first genotyped trough an hsp60 genepartial sequence as described previously (Goh, Potter, Wood, & Hemmingsen, 1996; Hunt et al., 2008) generating a total of 437 hsp60 sequences. MLST genotyping : Secondly, to validate the taxonomic affiliation of the isolates assigned to Harveyi clade, 3 additional protein-coding genes were sequenced (rctB, topA and mreB).