61da6771-38a5-4043-89af-87209ca51879 Goulais Maeva IFREMER Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Maeva.Goulais@ifremer.fr pointOfContact 2025-12-13T12:59:44.168421Z ISO 19115-3 - SEXTANT 1.0 WGS 84 (EPSG:4326) EPSG 8.6 eDNA sequencing of water, plastic and tissue of Pacific giant clam (Tridacna maxima) in a mesocosm approach for the P3M project, 2023 2023-05-25 creation 2024-04-01 publication FR-330-715-368-00032-IFR_BIOINFO_20230601_RMPF_MG_P3M_TRIDACNA_MAXIMA Here, our study aimed to first assess the influence of plastic on the bacterial community belonging to water, plastic and the microbiome of the giant clam and on the organism's physiology of this putative sentinel species. Our second objective was to identify bacteria whose abundance varies significantly with plastic concentration. Overall, this study will fill the gap towards a better understanding of the impact of plastic pollution on bacterial community assemblages in both inert and living environments. Ifremer (RMPF) IFREMER sismer@ifremer.fr publisher Goulais Maeva IFREMER Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Maeva.Goulais@ifremer.fr author Benestan Laura IFREMER Laura.Benestan@ifremer.fr author Darinot Thomas Sophie IFREMER sophie.darinot.thomas@ifremer.fr author Saulnier Denis IFREMER +689 40 54 60 52 +689 40 54 60 99 Centre Océanologique du Pacifique - Vairao - BP 49 - 98179 Taravao - Tahiti - Polynésie Française Tahiti France Denis.Saulnier@ifremer.fr author Galgani Francois IFREMER +689 40 54 60 87 +689 40 54 60 99 Centre Océanologique du Pacifique - Vairao - BP 49 - 98179 Taravao - Tahiti - Polynésie Française Tahiti France Francois.Galgani@ifremer.fr author Mitta Guillaume IFREMER Centre Océanologique du Pacifique - Vairao - BP 49 - 98179 Taravao - Tahiti - Polynésie Française Tahiti France Guillaume.Mitta@ifremer.fr author Rouxel Julien IFREMER Centre Océanologique du Pacifique - Vairao - BP 49 - 98179 Taravao - Tahiti - Polynésie Française Tahiti France Julien.Rouxel@ifremer.fr author Chevalier Oriane IFREMER oriane.chevalier@ifremer.fr author Basset Caline IFREMER Centre Océanologique du Pacifique - Vairao - BP 49 - 98179 Taravao - Tahiti - Polynésie Française Tahiti France Caline.Basset@ifremer.fr author Fleurot Baptiste IFREMER Baptiste.Fleurot@ifremer.fr author https://sextant.ifremer.fr/geonetwork/srv/api/records/61da6771-38a5-4043-89af-87209ca51879/attachments/BioinfoMarine.png Habitats and biotopes GEMET - INSPIRE themes, version 1.0 2018-07-27T14:39:48 publication 2018-07-27 publication geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme /Biological Environment/Bioinformatics Thèmes Sextant 2025-12-09 publication geonetwork.thesaurus.local.theme.sextant-theme Variables ODATIS 2023-10-13 publication geonetwork.thesaurus.local.theme.odatis_variables Bioinformatics ODATIS aggregation parameters and Essential Variable names 2025-07-29 publication geonetwork.thesaurus.external.theme.NVS.OD1 Analysis data Type de jeux de donnée ODATIS 2025-12-09 publication geonetwork.thesaurus.local.theme.type_jeux_donnee CC-BY (Creative Commons - Attribution) utf8 environment -180.00 180.00 -90.00 90.00 2022-07-17 2022-09-13 /home/ref-bioinfo-public/ifremer/rmpf/P3M NETWORK:LINK https://data-dataref.ifremer.fr/bioinfo/ifremer/rmpf/P3M WWW:DOWNLOAD Download link https://www.ebi.ac.uk/ena/browser/view/PRJEB74057 WWW:LINK Publication ENA https://doi.org/10.12770/61da6771-38a5-4043-89af-87209ca51879 DOI Digital Object Identifier (DOI) This study aimed to evaluate the impact of incrementally increasing plastic concentrations on the bacterial compartment in marine environments. We reproduced simplified tropical ecosystems in 9 seawater mesocosms, then immersed different quantities of macroplastics from pearl farm (copolymers of PP and PE). We then monitored the bacterial communities in three compartments: the water column, the Tridacna maxima and the plastic biofilm to identify the threshold at which modifications in the bacterial compartment occur. The objective was to identify early markers among impacted bacteria, indicative of potential risks exceeding critical thresholds for marine microbial fauna. Despite finding no influence of plastic concentration on bacterial diversity, significant impacts were observed for bacterial assemblages based on time and sample type. Digging deeper, our analysis at the individual bacterial scale revealed that at the highest plastic concentration (5.46g/L), a positive correlation was observed for 29 bacterial genera. This suggests that certain bacterial genera thrive at extreme concentrations of plastic, leading to an overabundance of these specific bacteria referred to here as candidate ASVs. The overabundance of candidate ASVs in ours mesocosms could be attributed to imbalanced competition relationships between bacteria, favouring genera less sensitive to environmental pollutants. Some of these bacteria have known ecological functions, including plastic degradation and pathogenicity, aligning with prior studies warning of the dangers associated with macroplastics in water, potentially promoting the emergence of pathogenic families by serving as substrates and modifying microbial interactions.