268e2e3a-cc78-4bda-835c-8feeaeb1922a Nunes Flavia IFREMER 02 98 22 49 27 02 98 22 45 48 Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Flavia.Nunes@ifremer.fr pointOfContact 2026-09-11T06:49:03.253003Z ISO 19115-3 - SEXTANT 1.0 WGS 84 (EPSG:4326) EPSG 8.6 Transcriptomic response of the honeycomb worm, Sabellaria alveolata, to temperature stress 2014-01-01 publication FR-330-715-368-00032-IFR_BIOINFO_SABELLARIA_ALVEOLATA_5_POPULATIONS DOI:10.12770/268e2e3a-cc78-4bda-835c-8feeaeb1922a Individuals from five populations of Sabellaria alveolata (Scotland, France-Saint Michel Bay, France-Biarritz, Portugal, and Morocco) were maintained under common garden conditions to investigate the gene expression response to temperature stress in the honeycomb worm. Worms were exposed to five sequential temperature treatments, and whole-organism samples were collected for RNA-Seq analysis. Gene expression patterns were analyzed to assess thermal acclimation and adaptation across populations. This work was conducted as part of the LabexMer International Chair in Evolutionary Marine Ecology, awarded to F. Nunes. It was supported by a grant from the Regional Council of Brittany, from the European Funds (ERDF) and supported by the “Laboratoire d’Excellence” LabexMER (ANR-10- LABX-19) and co-funded by a grant from the French government under the program “Investissements d’Avenir” IFREMER sismer@ifremer.fr publisher Nunes Flavia IFREMER 02 98 22 49 27 02 98 22 45 48 Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané Brest France Flavia.Nunes@ifremer.fr author Muir Anna Université de Bretagne Occidentale anna.muir@yahoo.co.uk author Dubois Stanislas IFREMER PDG-DGDS-ODE-DYNECO-LEBCO 02 98 22 49 18 Stanislas.Dubois@ifremer.fr author Pernet Fabrice IFREMER PDG-DGDS-RBE-PHYTNESS 02 98 22 46 37 Fabrice.Pernet@ifremer.fr author Moreau Yannis IFREMER Yannis.Moreau@ifremer.fr author https://sextant.ifremer.fr/geonetwork/srv/api/records/268e2e3a-cc78-4bda-835c-8feeaeb1922a/attachments/BioinfoMarine.png Habitats and biotopes GEMET - INSPIRE themes, version 1.0 2018-07-27T14:39:48 publication 2018-07-27 publication geonetwork.thesaurus.external.theme.httpinspireeceuropaeutheme-theme /Biological Environment/Bioinformatics Thèmes Sextant 2025-12-09 publication geonetwork.thesaurus.local.theme.sextant-theme Variables ODATIS 2023-10-13 publication geonetwork.thesaurus.local.theme.odatis_variables Bioinformatics ODATIS aggregation parameters and Essential Variable names 2025-07-29 publication geonetwork.thesaurus.external.theme.NVS.OD1 Analysis data Type de jeux de donnée ODATIS 2025-12-09 publication geonetwork.thesaurus.local.theme.type_jeux_donnee Données en accès restreint utf8 environment -180 180 -90 90 /home/ref-bioinfo-public/ifremer/dyneco/salveolata-heat NETWORK:LINK https://doi.org/10.12770/268e2e3a-cc78-4bda-835c-8feeaeb1922a DOI DOI of the dataset DOI of the dataset https://www.ebi.ac.uk/ena/browser/view/PRJEB86898 WWW:LINK ENA Publication https://data-dataref.ifremer.fr/bioinfo/ifremer/dyneco/salveolata-heat/ WWW:DOWNLOAD Download link Sabellaria alveolata is a reef-building polychaete that inhabits the rocky intertidal zone of Europe. As an intertidal species, S. alveolata has likely developed adaptations to cope with wide temperature fluctuations, being exposed to both air and sea surface temperatures that vary daily and seasonally across its range. To investigate the ability of the honeycomb worm to respond to temperature stress, individuals from five geographically distinct populations spanning the species' distribution (Scotland, English Channel coast of France, southern Atlantic coast of France, Portugal and Morocco) were collected and maintained under controlled common garden conditions. After one month of acclimation at 15°C (T0), temperature was increased by 1°C per day until reaching 20°C (T1), followed by a four-week acclimation period (T2). A second temperature challenge was then conducted by increasing the temperature by 1°C per day to 25°C (T3), followed by another four-week acclimation period (T4). Whole organisms were collected at experimental ssampling time point (T0-T4) from each population and placed in Trizol (Life Technologies), followed by flash freezing in liquid nitrogen. RNA was extracted with chloroform and percipitated in ethanol. RNA quality was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies,), and was quantified using the Quant-IT RiboGreen RNA Assay Kit (Life Technologies). Library preparation and sequencing were performed at Christian-Albrechts-Universität, Kiel, Germany, using an Illumina HiSeq2000 platform in October 2014. Paired-end sequencing (2 × 100 bp) was conducted to generate high-quality transcriptomic data.