seanoe:107654 ALBOUY Camille ETH Zürich, Switzerland Camille.Albouy@ifremer.fr 2025-10-24 ISO 19115:2003/19139 1.0 Environmental DNA data for Dicentrarchus labrax, Merluccius merluccius and Pagellus bogaraveo in the Bay of Biscay 2025-07 2025-10-24 Albouy Camille, Trenkel Verena (2025). Environmental DNA data for Dicentrarchus labrax, Merluccius merluccius and Pagellus bogaraveo in the Bay of Biscay. SEANOE. https://doi.org/10.17882/107654 In October 2019 we chose 15 sites from the 2019 EVHOE survey for environmental DNA (eDNA) sampling. The French international EVHOE bottom trawl survey is carried out annually during autumn in the BoB to monitor demersal fish resources. At each site, we sampled seawater using Niskin bottles deployed with a circular rosette. There were nine bottles on the rosette, each of them able to hold ∼5 l of water. At each site, we first cleaned the circular rosette and bottles with freshwater, then lowered the rosette (with bottles open) to 5 m above the sea bottom, and finally closed the bottles remotely from the boat. The 45 l of sampled water was transferred to four disposable and sterilized plastic bags of 11.25 l each to perform the filtration on-board in a laboratory dedicated to the processing of eDNA samples. To speed up the filtration process, we used two identical filtration devices, each composed of an Athena® peristaltic pump (Proactive Environmental Products LLC, Bradenton, Florida, USA; nominal flow of 1.0 l min–1 ), a VigiDNA 0.20 μm filtration capsule (SPYGEN, le Bourget du Lac, France), and disposable sterile tubing. Each filtration device filtered the water contained in two plastic bags (22.5 l), which represent two replicates per sampling site. We followed a rigorous protocol to avoid contamination during fieldwork, using disposable gloves and single-use filtration equipment and plastic bags to process each water sample. At the end of each filtration, we emptied the water inside the capsule that we replaced by 80 ml of CL1 conservation buffer and stored the samples at room temperature following the specifications of the manufacturer (SPYGEN, Le Bourget du Lac, France). We processed the eDNA capsules at SPYGEN, following the protocol proposed by Polanco-Fernández et al., (2020). Half of the extracted DNA was processed by Sinsoma using newly developped ddPCR assays for European seabass (Dicentrachus labrax), European hake (Merluccius merluccius) and blackspot seabream (Pagellus bogaraveo).  The other half of the extracted DNA was analysed using metabarcoding with teleo primer. The raw metabarcoding data set is available at https://www.doi.org/10.16904/envidat.442 Bottom trawling using a GOV trawl was carried out before or after water sampling. The catch was sorted by species and catches in numbers and weight were recorded. No blackspot seabream individuals were caught.   Data content: * ddPCR/: contains the ddPCR counts and DNA concentrations for each sample and species. * SampleInfo/: contains the filter volume for each eDNA sample. * StationInfo/: contains metadata related to the data collected in the field for each filter. * Metabarcoding/: contains metabarcoding results for teleoprimer. * Trawldata/: contains catch data in numbers and weight (kg).       <p>The sample collection and molecular analysis was funded by Ifremer.</p> ALBOUY Camille ETH Zürich, Switzerland Camille.Albouy@ifremer.fr TRENKEL Verena UMR DECOD, Ifremer, France SEANOE https://www.seanoe.org/data/00965/107654/illustration.jpg Environmental DNA concentrations (ddPCR) for Dicentrarchus labrax in 2019. eDNA ddPCR Bay of Biscay hake blackspot seabream seabass metabarcoding catch Fisheries and aquaculture Biological oceanography Bioinformatics Fish ODATIS aggregation parameters and Essential Variable names 2025-07-29 geonetwork.thesaurus.external.theme.NVS.OD1 Fisheries and aquaculture Biological oceanography SeaDataNet Parameter Disciplines 2025-07-29 geonetwork.thesaurus.external.parameter.NVS.P08 /Observational data/in-situ Type de jeux de donnée ODATIS 2024-10-15 geonetwork.thesaurus.local.theme.type_jeux_donnee CC-BY (Creative Commons - Attribution) 10.1002/edn3.70187 10.17600/18000878 oceans -5.537109 -0.131836 42.779275 48.107431 CSV https://www.seanoe.org/data/00965/107654/data/120983.csv WWW:DOWNLOAD-1.0-link--download Raw data StationInfo_Evhoe2019 - 1 KB https://www.seanoe.org/data/00965/107654/data/120984.csv WWW:DOWNLOAD-1.0-link--download Processed data ddPCR_Evhoe2019 - 40 KB https://www.seanoe.org/data/00965/107654/data/120985.csv WWW:DOWNLOAD-1.0-link--download Processed data Metabarcoding_Evhoe2019 - 3 KB https://www.seanoe.org/data/00965/107654/data/120986.csv WWW:DOWNLOAD-1.0-link--download Raw data SampleInfo_Evhoe2019 - 1023 bytes https://www.seanoe.org/data/00965/107654/data/120987.csv WWW:DOWNLOAD-1.0-link--download Quality controlled data Catch_Evhoe2019 - 5 KB https://doi.org/10.17882/107654 WWW:LINK-1.0-http--metadata-URL DOI of the product https://www.seanoe.org/data/00965/107654/ rel-canonical Seanoe Niskin bottles, filter capsules and species-specific assays for environmental DNA (ddPCR and metabarcoding) GOV bottom trawl