seanoe:79823 Egge Elianne University of Oslo, Department of Biosciences, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY University of Duisburg-Essen, Fakultät für Biologie, Universitätsstr. 5, DE-45141 Essen, GERMANY 2021-05-12 ISO 19115:2003/19139 1.0 An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth. 2014 2021-05-12 Egge Elianne, Elferink Stephanie, Vaulot Daniel, John Uwe, Bratbak Gunnar, Larsen Aud, Edvardsen Bente (2014). An 18S V4 rDNA metabarcoding dataset of protist diversity in the Atlantic inflow to the Arctic Ocean, through the year and down to 1000 m depth. SEANOE. https://doi.org/10.17882/79823 Arctic marine protist communities have been understudied due to challenging sampling conditions, in particular during winter and in deep waters. The aim of this study was to improve our knowledge on Arctic protist diversity through the year, both in the epipelagic (< 200 m depth)  and mesopelagic zones (200-1000 m depth). Sampling campaigns were performed in 2014, during five different months, to capture the various phases of the Arctic primary production: January (winter), March (pre-bloom), May (spring bloom), August (post-bloom) and November (early winter). The cruises were undertaken west and north of the Svalbard archipelago, where warmer Atlantic waters from the West Spitsbergen Current meets cold Arctic waters from the Arctic Ocean. From each cruise, station, and depth, 50 L of sea water were collected and the plankton was size-fractionated by serial filtration into four size fractions between 0.45-200 µm, representing the picoplankton, nanoplankton and microplankton. In addition vertical net hauls were taken from 50 m depth to the surface at selected stations. From the plankton samples DNA was extracted, the V4 region of the 18S rRNA-gene was amplified by PCR with universal eukaryote primers and the amplicons were sequenced by Illumina high-throughput sequencing. Sequences were clustered into Amplicon Sequence Variants (ASVs), representing protist genotypes, with the dada2 pipeline. Taxonomic classification was made against the curated Protist Ribosomal Reference database (PR2). Here we present the ASV table, metadata for the sequenced size-fractionated plankton samples, and environmental data from the corresponding water masses. Fastq-files with raw reads are available from the European Nucleotide Archive, under project accession number PRJEB40133. The study was conducted as part of the Research Council of Norway supported project Micropolar - Processes and Players in Arctic Marine Pelagic Food Webs - Biogeochemistry, Environment and Climate Change no. 225956/E10 (prosjektbanken.forskningsradet.no/#/project/NFR/225956/). DV was supported by ANR contract PhytoPol (ANR-15-CE02-0007). We wish to thank members of the MicroPolar and Carbon Bridge projects for assisting in the sampling campaigns, and the crews at K/V Svalbard (January cruise), R/V Lance (March) and R/V Helmer Hanssen (May, August and November cruise) Egge Elianne University of Oslo, Department of Biosciences, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY University of Duisburg-Essen, Fakultät für Biologie, Universitätsstr. 5, DE-45141 Essen, GERMANY Elferink Stephanie Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung, Am Handelshafen 12, DE-27570 Bremerhaven, GERMANY Vaulot Daniel UMR7144, CNRS, Sorbonne Université, Station Biologique de Roscoff. Place Georges Teissier, FR-29682 Roscoff, FRANCE Asian School of the Environment, Nanyang Technological University, 50 Nanyang Avenue, SINGAPORE 639798 John Uwe Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung, Am Handelshafen 12, DE-27570 Bremerhaven, GERMANY Bratbak Gunnar University of Bergen, Department of Biological Sciences, PO Box 7803, NO-5020 Bergen, NORWAY Larsen Aud NORCE Norwegian Research Centre AS, PO Box 7810, NO-5020 Bergen, NORWAY Edvardsen Bente University of Oslo, Department of Biosciences, PO Box 1066 Blindern, NO-0316 Oslo, NORWAY SEANOE https://www.seanoe.org/data/00686/79823/illustration.gif Map of MicroPolar sampling stations protists metabarcoding Arctic 18s rDNA microbial eukaryotes epipelagic zone mesopelagic zone seasonality winter spring bloom post bloom West Spitsbergen Current Svalbard Protist Ribosomal Reference Database picoplankton nanoplankton microplankton size fractions Bioinformatics ODATIS aggregation parameters and Essential Variable names 2025-07-29 geonetwork.thesaurus.external.theme.NVS.OD1 CDS-STAMAR Centre de données ODATIS 2026-01-19 geonetwork.thesaurus.local.theme.odatis_centre_donnees /Observational data/in-situ Type de jeux de donnée ODATIS 2025-12-09 geonetwork.thesaurus.local.theme.type_jeux_donnee CC-BY (Creative Commons - Attribution) CarbonBridge 2014: Physical oceanography and microorganism composition during 5 cruises (Jan, March, May, August, Nov 2014) on and off the shelf northwest of Svalbard in 2014 2017 PANGAEA Paulsen Maria Lund Bratbak Gunnar Larsen Aud Seuthe Lena Egge Jorun K Erga Svein Rune 10.1594/pangaea.884255 oceans -4.96582 20.961915 75.982314 83.29221 XLS, XLSX TEXTE https://www.seanoe.org/data/00686/79823/data/82625.xlsx WWW:DOWNLOAD-1.0-link--download Processed data ASV table of an 18s rDNA metabarcoding dataset of arctic marine protists. - 4 MB https://www.seanoe.org/data/00686/79823/data/82626.txt WWW:DOWNLOAD-1.0-link--download Meta data for the sequenced DNA samples in the ASV table. - 30 KB https://www.seanoe.org/data/00686/79823/data/82627.txt WWW:DOWNLOAD-1.0-link--download Raw data Environmental data from the water masses corresponding to the size-fractionated plankton samples. - 23 KB https://doi.org/10.17882/79823 DOI DOI of the product https://www.seanoe.org/data/00686/79823/ rel-canonical Seanoe