• Catalogue PIGMA
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SARS-CoV-2 whole genome sequencing in wastewaters

WGS of SARS-CoV-2 by Oxford Nanopore Technology from raw wastewater samples collected in France, 2020-2021

Simple

Date (Creation)
2021-01-01
Date (Publication)
2022-02-02
Identifier
FR-330-715-368-00032-IFR_BIOINFO_SEQ_SARS_OBEPINE
Identifier
DOI:10.12770/90a96749-8fa1-467d-9af8-bd4ab1ae48bd
Credit
Ifremer - Laboratoire Sante Environnement Microbiologie
Publisher
  IFREMER
Author
  Laboratoire de Microbiologie, LSEM, Ifremer. - Barbe Laure
Centre Atlantique - Rue de l'Ile d'Yeu - BP 21105 - 44311 Nantes Cedex 03 , Nantes , France
02 40 37 43 33
Author
  Laboratoire de Microbiologie, LSEM, Ifremer. - Schaeffer Julien
Author
  Laboratoire de Microbiologie, LSEM, Ifremer. - Besnard Alban
Author
  Laboratoire de Microbiologie, LSEM, Ifremer. - Jousse Sarah
Author
  Scientific interest group Obépine
Author
  Laboratoire de Microbiologie, LSEM, Ifremer. - Françoise S Le Guyader
Author
  Laboratoire de Microbiologie, LSEM, Ifremer. - Desdouits Marion
GEMET - INSPIRE themes, version 1.0
  • Habitats and biotopes
Thèmes Sextant
  • /Biological Environment/Bioinformatics
ODATIS aggregation parameters and Essential Variable names
  • Bioinformatics
Use limitation
CC-BY (Creative Commons - Attribution)
Access constraints
License
Use constraints
License
Spatial representation type
grid Grid
Metadata language
English
Character set
utf8 UTF8
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S
E
W
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Begin date
2021-01-01
End date
2021-12-31
Reference system identifier
EPSG / WGS 84 (EPSG:4326) / 8.6

Spatial representation info

No information provided.
Number of dimensions
0
Cell geometry
Area
Distribution format
  • ( )

OnLine resource
/home/ref-bioinfo/ifremer/obepine ( NETWORK:LINK )
OnLine resource
Lien de téléchargement ( WWW:DOWNLOAD )

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OnLine resource
Digital Object Identifier (DOI) ( DOI )
Hierarchy level
Dataset
Statement
Raw wastewaters (WW) were collected weekly from two WW treatment plants in a city in western France. A subsample was ultracentrifuged, nucleic acids axtracted from the pellet, RNA reverse-transcribed and submitted to an optimized ARTIC400 V3 PCR. PCR products were purified, barcoded and adapter-ligated using Oxford Nanopore Technology kits, and libraries sequenced on an R9.4 flow cells using a Mk1c MinION. Results from 6 runs are shared as raw data (fast5 files) and based-called data using Guppy 4.3.4 (fastq files).
File identifier
90a96749-8fa1-467d-9af8-bd4ab1ae48bd XML
Metadata language
English
Character set
UTF8
Hierarchy level
Dataset
Date stamp
2025-05-15T23:19:16.014581Z
Metadata standard name
ISO 19115-3 - SEXTANT ISO 19115-3 - SEXTANT
Metadata standard version
1.0
Point of contact
  IFREMER - Desdouits Marion
Station de Palavas - Chemin de Maguelone - 34250 Palavas les Flots , Palavas , France
04 67 13 04 14
 
 

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S
E
W
thumbnail


Keywords

GEMET - INSPIRE themes, version 1.0
Habitats and biotopes
ODATIS aggregation parameters and Essential Variable names
Bioinformatics
Thèmes Sextant
/Biological Environment/Bioinformatics

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