Date (Creation)

2020-03-02

Date (Publication)

2024-01-24

Identifier

FR-330-715-368-00032-IFR_BIOINFO_METABA_SHRIMP_HEMOLYMPH

Credit

Projet COMISTYL

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Thèmes Sextant

• /Biological Environment/Bioinformatics

ODATIS aggregation parameters and Essential Variable names

• Bioinformatics

Type de jeux de donnée ODATIS

• Analysis data

Use limitation

CC-BY (Creative Commons - Attribution)

Access constraints

License

Use constraints

License

Spatial representation type

grid Grid

Metadata language

English

Character set

utf8 UTF8

Topic category

• Oceans

Begin date

2019-03-07

End date

2019-10-17

Reference system identifier

EPSG / WGS 84 (EPSG:4326) / 8.6

Distribution format

• ( )

OnLine resource

/home/ref-bioinfo/ifremer/leadnc/comistyl ( NETWORK:LINK )

Hierarchy level

Dataset

Statement

Sequencing data for this project were acquired from extraction of total RNA from the hemolymph of Penaeus stylirostris shrimp and from the water of their environment. The shrimp were reared under contrasting conditions: two rearing waters (earthen pond and clear water tanks), at two sampling times (beginning of the experiment, 9 months old shrimp reared in earthen ponds then partially transferred to clear water tanks and reared in both waters in parallel for one month, end of the experiment), during two seasons (warm and cold) and on two sites (New Caledonia and French Polynesia). RNA extractions in hemolymph samples were performed with the Direct-Zol Mini-Prep kit (Zymo Research) according to the manufacturer's protocol. RNA extractions in water samples were performed according to the manufacturer's protocol with the RNeasy PowerWater kit (Qiagen). The extracted RNAs were reverse transcribed into cDNA by M-MLV reverse transcriptase (PROMEGA) in a thermocycler (Verity TM, Biosystems). Finally, all cDNAs were sequenced by MrDNA (Shallowater, TX, Uited Sates) with primers 515F/806Rs on Illumina HiSeq, 2 x 300 bases and an average sequencing depth of 20,000 reads.

File identifier

edd1b589-b654-46e6-ba6a-493eb2b9ec82 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Non geographic dataset

Date stamp

2025-12-13T10:08:35.456418Z

Metadata standard name

ISO 19115-3 - SEXTANT

Metadata standard version

1.0