Date (Creation)

2025-09-09

Date (Publication)

2025-12-16

Identifier

FR-330-715-368-00032-IFR_BIOINFO_CASPAR

Credit

Ifremer - Laboratoire Ecosystemes microbiens et molecules marines pour les biotechnologies - FOODGUARD Project

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Thèmes Sextant

• /Biological Environment/Bioinformatics

ODATIS aggregation parameters and Essential Variable names

• Bioinformatics

Type de jeux de donnée ODATIS

• /Observational data/in-situ

Use limitation

CC-BY (Creative Commons - Attribution)

Access constraints

Restricted

Use constraints

Restricted

Metadata language

Français

Character set

utf8 UTF8

Topic category

• Environment

Distribution format

• ( )

OnLine resource

Télécharger (chemin visible) ( NETWORK:LINK )

OnLine resource

ENA Publication (embargo) ( WWW:LINK )

OnLine resource

Digital Object Identifier (DOI) ( DOI )

Hierarchy level

Dataset

Statement

The Mediterranean gilthead seabream (Sparus aurata) is a widely consumed aquaculture product, with global production reaching 354,920 tonnes in 2022 (FAO, 2024). However, it is highly perishable and prone to microbial spoilage, leading to significant food waste. After slaughter, the flesh becomes rapidly colonized by microorganisms, some of which cause quality deterioration. Biopreservation, a mild preservation method, aims to control food microbiota by introducing microorganisms or metabolites with antimicrobial activity (Passerini et al., 2021). Within the framework of the European FOODGUARD project, five protective lactic acid bacteria (LAB) strains were tested on seabream fillets. During CT1 and CT2, the fillets were stored under modified atmosphere packaging at 8 °C for 11 days. The effects of bioprotective strains on microbial communities and product quality were evaluated through two initial trials (CT1 and CT2). Based on these results, one protective culture was selected and further tested in another challenge-test (CT3) at 0, 4, and 8 °C for up to 19 days, with samples analyzed once again by DNA extraction and metabarcoding. In a fourth trial (CT4), the one selected protective lactic acid bacteria strain (PC4) was tested on fillets stored at 8°C under two modified atmosphere packaging (MAP) conditions: MAP 1 (20% O₂ / 50% CO₂ / 30% N₂) or MAP 2 (50% CO₂ / 50% N₂). The 16S rRNA gene Illumina sequencing (V3–V4 regions) was used to characterize the dynamics of microbial communities across the different trials and to assess the impact of protective cultures on the microbiota composition of seabream fillets.

File identifier

f1300407-194a-4ebe-acf0-fe8599b81e66 XML

Metadata language

English

Character set

UTF8

Hierarchy level

Non geographic dataset

Date stamp

2026-05-28T07:49:54.709052Z

Metadata standard name

ISO 19115-3 - SEXTANT ISO 19115-3 - SEXTANT

Metadata standard version

1.0