• Catalogue PIGMA
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Metabarcoding in the Thau and Berre lagoons to detect Veneroidaee

In the framework of the PALDIAG project, environmental DNA from seawater was gathered in 2023 in Thau and Berre lagoons and studied with a 16S marker in order to detect the Veneroidae species.

Simple

Date (Creation)
2023-11-08
Date (Publication)
2024-01-29
Identifier
FR-330-715-368-00032-IFR_BIOINFO_paldiag_20231123_MARBEC_SL

Identifier

No information provided.
Credit
IFREMER (MARBEC/LEGPM)
Publisher
  IFREMER
Author
  IFREMER - Lapegue Sylvie
Université de Montpellier - Campus Triolet - 2 Place E. Bataillon - CC080 (IHPE) - 34095 Montpellier Cedex 5 , Montpellier , France
Author
  IFREMER - Fortunato Honoré
Author
  IFREMER - Cornette Florence
Station de Sète - Avenue Jean Monnet - CS 30171 - 34203 Sète Cedex , Sete , France
04 99 57 32 84
Author
  Corinne.Bouvier@cnrs.fr - Bouvier Corinne
Author
  Herve.Violette@ifremer.fr - Violette Hervé
Author
  Argaly - Bonin Aurélie
Author
  IFREMER - Noel Cyril
Centre Bretagne - ZI de la Pointe du Diable - CS 10070 - 29280 Plouzané , Brest , France
02 98 22 43 34
Author
  IFREMER - Lagarde Franck
Station de Sète - Avenue Jean Monnet - CS 30171 - 34203 Sète Cedex , Sete , France
04 99 57 32 90
GEMET - INSPIRE themes, version 1.0
  • Habitats and biotopes
Thèmes Sextant
  • /Biological Environment/Bioinformatics
ODATIS aggregation parameters and Essential Variable names
  • Bioinformatics
Use limitation
CC-BY (Creative Commons - Attribution)
Access constraints
License
Use constraints
License
Spatial representation type
grid Grid
Metadata language
English
Character set
utf8 UTF8
Topic category
  • Oceans
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E
W
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Begin date
2023-06-01
End date
2023-07-31
Reference system identifier
EPSG / WGS 84 (EPSG:4326) / 8.6

Spatial representation info

No information provided.
Distribution format
  • ( )

OnLine resource
/home/ref-bioinfo/ifremer/marbec/paldiag ( NETWORK:LINK )
OnLine resource
Digital Object Identifier (DOI) ( DOI )
Hierarchy level
Dataset
Statement
Seawater was filtered in June-July 2023 in Thau and Berre lagoons. The filtering capsules (24) are Waterra systems using a customized pumping system commercialized by Argaly company. DNA was resuspended in the capsule vigorous agitation after the injection of Longmire buffer, and extracted at Argaly. Whole DNA was used to construct libraries of metabarcoding using 16S primers specific to Veneroidae. Sequencing was performed on a MiSeq sequencer (2X 150 bp). Besides an experiment was conducted in October 2022 in Ifremer Sète and 14 additionnal capsules were obtained from the filtering of water in aquariums filled with different ratio of two clam species.
File identifier
76488bcc-e2c7-4839-a28e-978a2e6eee81 XML
Metadata language
English
Character set
UTF8
Hierarchy level
Dataset
Date stamp
2025-05-15T22:55:04.849601Z
Metadata standard name
ISO 19115-3 - SEXTANT
Metadata standard version
1.0
Point of contact
  IFREMER - Lapegue Sylvie
Université de Montpellier - Campus Triolet - 2 Place E. Bataillon - CC080 (IHPE) - 34095 Montpellier Cedex 5 , Montpellier , France
 
 

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N
S
E
W
thumbnail


Keywords

GEMET - INSPIRE themes, version 1.0
Habitats and biotopes
ODATIS aggregation parameters and Essential Variable names
Bioinformatics
Thèmes Sextant
/Biological Environment/Bioinformatics

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