Date (Publication)

2014-01-01

Identifier

FR-330-715-368-00032-IFR_BIOINFO_SABELLARIA_ALVEOLATA_5_POPULATIONS

Identifier

DOI:10.12770/268e2e3a-cc78-4bda-835c-8feeaeb1922a

Credit

This work was conducted as part of the LabexMer International Chair in Evolutionary Marine Ecology, awarded to F. Nunes. It was supported by a grant from the Regional Council of Brittany, from the European Funds (ERDF) and supported by the “Laboratoire d’Excellence” LabexMER (ANR-10- LABX-19) and co-funded by a grant from the French government under the program “Investissements d’Avenir”

GEMET - INSPIRE themes, version 1.0

• Habitats and biotopes

Thèmes Sextant

• /Biological Environment/Bioinformatics

ODATIS aggregation parameters and Essential Variable names

• Bioinformatics

Type de jeux de donnée ODATIS

• Analysis data

Use limitation

Données en accès restreint

Access constraints

Restricted

Use constraints

Restricted

Metadata language

English

Character set

utf8 UTF8

Topic category

• Environment

Reference system identifier

EPSG / WGS 84 (EPSG:4326) / 8.6

Distribution format

• ( )

OnLine resource

/home/ref-bioinfo-public/ifremer/dyneco/salveolata-heat ( NETWORK:LINK )

OnLine resource

DOI of the dataset ( DOI )

DOI of the dataset

OnLine resource

ENA Publication ( WWW:LINK )

OnLine resource

Download link ( WWW:DOWNLOAD )

Hierarchy level

Dataset

Statement

Sabellaria alveolata is a reef-building polychaete that inhabits the rocky intertidal zone of Europe. As an intertidal species, S. alveolata has likely developed adaptations to cope with wide temperature fluctuations, being exposed to both air and sea surface temperatures that vary daily and seasonally across its range. To investigate the ability of the honeycomb worm to respond to temperature stress, individuals from five geographically distinct populations spanning the species' distribution (Scotland, English Channel coast of France, southern Atlantic coast of France, Portugal and Morocco) were collected and maintained under controlled common garden conditions. After one month of acclimation at 15°C (T0), temperature was increased by 1°C per day until reaching 20°C (T1), followed by a four-week acclimation period (T2). A second temperature challenge was then conducted by increasing the temperature by 1°C per day to 25°C (T3), followed by another four-week acclimation period (T4). Whole organisms were collected at experimental ssampling time point (T0-T4) from each population and placed in Trizol (Life Technologies), followed by flash freezing in liquid nitrogen. RNA was extracted with chloroform and percipitated in ethanol. RNA quality was verified using an Agilent 2100 Bioanalyzer (Agilent Technologies,), and was quantified using the Quant-IT RiboGreen RNA Assay Kit (Life Technologies). Library preparation and sequencing were performed at Christian-Albrechts-Universität, Kiel, Germany, using an Illumina HiSeq2000 platform in October 2014. Paired-end sequencing (2 × 100 bp) was conducted to generate high-quality transcriptomic data.

File identifier

268e2e3a-cc78-4bda-835c-8feeaeb1922a XML

Metadata language

English

Character set

UTF8

Hierarchy level

Non geographic dataset

Date stamp

2026-09-11T06:49:03.253003Z

Metadata standard name

ISO 19115-3 - SEXTANT

Metadata standard version

1.0