WGS of SARS-CoV-2 by Oxford Nanopore Technology from raw wastewater samples collected in France, 2020-2021

Genetic diversity is often considered a key factor in a species’ ability to adapt to environmental changes and our aim was to understand the biological, ecological, and biogeographic factors that explain differences in genetic diversity among marine fish. Therefore, whole genomes of 160 individual marine fish were sequenced to a minimum depth of 15×, enabling near-complete representation of each diploid genome.

This dataset consists of metatranscriptomic sequencing reads corresponding to coastal micro-eukaryote communities sampled in Western Europe in 2018 and 2019.

The present data set concerne metabarcoding raw reads produced using 4 different PCR targeting polymerase or capside coding region of the genoyupe I and II of norovirus. Test samples of norovirus with serial dilutions in pure water and after a bio-accumulation in oysters. Sequencing was made after VirCapSeq-VERT approach.

scRNA-seq reads from a Pacific oyster (Crassostrea gigas) hemocyte preparation. Hemocytes were isolated from a unique immunologically naive animal (Ifremer Standardized Animal, 18 months) and single-cell drop-seq technology was applied to 3,000 individual hemocytes.

Comparison between 3 kits, Roche, Twist Biosciences and Illumina on the ability to enrich environmental samples to viral sequences. 5 sewage samples were extracted in triplicats with selective extraction protocol.

Vibrio bacteria sampled from juvenile oysters and seawater collected in Thau Lagoon (Languedoc-Roussillon, France) in October 2015 during a mortality event were genotyped using hsp60, rctB, topA and mreB protein-coding genes

DNA sequencing of Crassostrea gigas Pacific oyster spat infected in the wild with OsHV-1 virus in 4 French oyster basins (Marennes Oleron Bay, Arcachon bay, Rade de Brest and Thau lagoon).

Develop parentage assignment panels using genetic fingerprinting of pearl oysters for use in commercial hatcheries and research to manage pedigrees in order to limit the risks of loss of genetic variability and increased inbreeding of commercial lines.

This study aims to compare different metabarcoding sequences of commercially fished shrimps collected by tree counties on the North Brazil Shelf Large Marine Ecosystem