Bioinformatics
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Metabolome of of the marine diatom Haslea ostrearia. Bacteria were isolated from Haslea ostrearia isolates cultivated in ES 1/3 medium in laboratory conditions over a 3-month period. These microalgal isolates were recovered from four sites on the French Atlantic coast: Bouin , La Barre-de-Monts (46.90 N; 2.11°W), Isle de Ré (46.22 N; 1.45°W), and La Tremblade (45.80 N; 1.15°W) . Data processing and statistical analysis of the metabolic profiles were performed on an LC/MS Metabolomics Discovery Workflow using Mass Profiler Professional Software and an Agilent 1290 Infinity II LC system coupled to an Agilent 6540 UHD Accurate-Mass QTOF hybrid mass spectrometer (Agilent Technologies, Waldbronn, Germany) equipped with a dual electrospray ionization (ESI) source. The full history (tools, parameters, input and output data files) is publicly available on http://dx.doi.org/10.12770/046e1e6a-864e-48a6-944b-d8613d67de0f
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Metabarcoding data were produced based on samples gathered at Ifremer where the DNA was extracted; PCR libraries were built at Ifremer and Genseq; libraries were sequenced at Novogene. The data to download contain: 1/d emultiplexed raw data, 2/ metadata, and 3) Scripts to process data and taxonomically assign DNA sequences 4) Rmarkdown to analyze communities.
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Here, our study aimed to first assess the influence of plastic on the bacterial community belonging to water, plastic and the microbiome of the giant clam and on the organism's physiology of this putative sentinel species. Our second objective was to identify bacteria whose abundance varies significantly with plastic concentration. Overall, this study will fill the gap towards a better understanding of the impact of plastic pollution on bacterial community assemblages in both inert and living environments.
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Metagenomic analysis of clams from Sanaga river in Cameroon to describe the virome
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In the framework of the PALDIAG project, environmental DNA from seawater was gathered in 2023 in Thau and Berre lagoons and studied with a 16S marker in order to detect the Veneroidae species.
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Whole genome pooled sequencing of individuals from 4 populations and 3 different color phenotype in order to uncover the genetic variants linked to color expression in the pearl oyster P. margaritifera.
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DNA sequencing of Crassostrea gigas Pacific oyster spat infected in the wild with OsHV-1 virus in 4 French oyster basins (Marennes Oleron Bay, Arcachon bay, Rade de Brest and Thau lagoon).
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Successive infections with Vibrio harveyi were conducted in two populations of the European abalone in order to examine which genes may be involved in improved survival to the disease in the St. Malo population.
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Vibrio bacteria sampled from juvenile oysters and seawater collected in Thau Lagoon (Languedoc-Roussillon, France) in October 2015 during a mortality event were genotyped using hsp60, rctB, topA and mreB protein-coding genes
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Sequenced samples are city center wastewater sampled by passive samplers. Variants are identified by Illumina Miseq sequencing.