Bioinformatics
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2bRAD genotyping will be used to estimate genetic diversity and connectivity among populations of Sabellaria alveolata. We will relate population genetic parameters with reef state characteristics.
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ddRAD genotyping was used to evaluate population connectivity and putative loci under selection in honeycomb worm from 13 sites spanning its distribution in the Atlantic and Mediterranean coasts.
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The project was designed to explore biological rhythms in the hydrothermal vent mussel Bathymodiolus azoricus. The experiment provides the first high-resolution temporal transcriptomes of an hydrothermal species, both in situ and in the laboratory.
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Dart Seq data gathered on Blue Shark in the framework of the PSTBS-IO project supported by funding from FAO, CSIRO Oceans and Atmosphere, AZTI Tecnalia, Institut de recherche pour le développement (IRD), and Research Institute for Tuna Fisheries (RITF) and financial assistance of the European Union (GCP/INT/233/EC – Population structure of IOTC species in the Indian Ocean), and POPSIZE project supported by FEAMP (2014-2020 UE N°508/2014), and Institut français de recherche pour l'Exploitation de la mer (Ifremer).
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Land-sea continuum microbiome analyses in 4 coastal French sites and in oysters aimed at evaluating human impact on coastal ecosystems and new potentiel microbiological sanitary risks.
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Metagenomic analysis of clams from Sanaga river in Cameroon to describe the virome
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Sequenced samples are city center wastewater sampled by passive samplers. Variants are identified by Illumina Miseq sequencing.
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DNA sequencing of Crassostrea gigas Pacific oyster spat experimentally infected with OsHV-1 virus from oyster basin of Marennes-Oleron
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Metabarcoding data were produced based on samples gathered at Ifremer where the DNA was extracted; PCR libraries were built at Ifremer and Genseq; libraries were sequenced at Novogene. The data to download contain: 1/d emultiplexed raw data, 2/ metadata, and 3) Scripts to process data and taxonomically assign DNA sequences 4) Rmarkdown to analyze communities.
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scRNA-seq reads from a Pacific oyster (Crassostrea gigas) hemocyte preparation. Hemocytes were isolated from a unique immunologically naive animal (Ifremer Standardized Animal, 18 months) and single-cell drop-seq technology was applied to 3,000 individual hemocytes.