Bioinformatics
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WGS for Iatlantic projet ( ) for assessing past and present connectivity
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This dataset contains libraries of 3 coral species: Acropora hyacinthus, Porites lobata and Poscillopora acuta. In three islands with contrasting thermal regimes, the three species were sampled and brought back to the laboratory to induce an experimental thermal stress. The different colonies were split into two conditions. One part was placed in tanks filled with seawater at a given control temperature, the other part in tanks where the water temperature was increased. The samples in this dataset correspond to part of the control condition samples from French Polynesia and New Caledonia.
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Metabarcoding data were produced based on samples gathered at Ifremer where the DNA was extracted; PCR libraries were built at Ifremer and Genseq; libraries were sequenced at Novogene. The data to download contain: 1/d emultiplexed raw data, 2/ metadata, and 3) Scripts to process data and taxonomically assign DNA sequences 4) Rmarkdown to analyze communities.
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WGS of SARS-CoV-2 by Oxford Nanopore Technology from raw wastewater samples collected in France, 2020-2021
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Land-sea continuum microbiome analyses in 4 coastal French sites and in oysters aimed at evaluating human impact on coastal ecosystems and new potentiel microbiological sanitary risks.
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Metabolome of of the marine diatom Haslea ostrearia. Bacteria were isolated from Haslea ostrearia isolates cultivated in ES 1/3 medium in laboratory conditions over a 3-month period. These microalgal isolates were recovered from four sites on the French Atlantic coast: Bouin , La Barre-de-Monts (46.90 N; 2.11°W), Isle de Ré (46.22 N; 1.45°W), and La Tremblade (45.80 N; 1.15°W) . Data processing and statistical analysis of the metabolic profiles were performed on an LC/MS Metabolomics Discovery Workflow using Mass Profiler Professional Software and an Agilent 1290 Infinity II LC system coupled to an Agilent 6540 UHD Accurate-Mass QTOF hybrid mass spectrometer (Agilent Technologies, Waldbronn, Germany) equipped with a dual electrospray ionization (ESI) source. The full history (tools, parameters, input and output data files) is publicly available on http://dx.doi.org/10.12770/046e1e6a-864e-48a6-944b-d8613d67de0f
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The present data set concerne metabarcoding raw reads produced using 4 different PCR targeting polymerase or capside coding region of the genoyupe I and II of norovirus. Test samples of norovirus with serial dilutions in pure water and after a bio-accumulation in oysters. Sequencing was made after VirCapSeq-VERT approach.
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scRNA-seq reads from a Pacific oyster (Crassostrea gigas) hemocyte preparation. Hemocytes were isolated from a unique immunologically naive animal (Ifremer Standardized Animal, 18 months) and single-cell drop-seq technology was applied to 3,000 individual hemocytes.
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Metagenomic analysis of clams from Sanaga river in Cameroon to describe the virome
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Whole genome pooled sequencing of individuals from 4 populations and 3 different color phenotype in order to uncover the genetic variants linked to color expression in the pearl oyster P. margaritifera.