Bioinformatics
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Here, our study aimed to first assess the influence of plastic on the bacterial community belonging to water, plastic and the microbiome of the giant clam and on the organism's physiology of this putative sentinel species. Our second objective was to identify bacteria whose abundance varies significantly with plastic concentration. Overall, this study will fill the gap towards a better understanding of the impact of plastic pollution on bacterial community assemblages in both inert and living environments.
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Individuals from 5 populations were kept in common garden conditions in order to examine acclimation and adaptation to temperature in the honeycomb worm. Worms were exposed to 5 temperature treatments, and collected for RNAseq analysis. Gene expression patterns were then examined.
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Vibrio bacteria sampled from juvenile oysters and seawater collected in Thau Lagoon (Languedoc-Roussillon, France) in October 2015 during a mortality event were genotyped using hsp60, rctB, topA and mreB protein-coding genes
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Raw reads for the assembly of Gambusia holbrooki genome.
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WGS of SARS-CoV-2 by Oxford Nanopore Technology from raw wastewater samples collected in France, 2020-2021
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DNA sequencing of Crassostrea gigas Pacific oyster spat infected in the wild with OsHV-1 virus in 4 French oyster basins (Marennes Oleron Bay, Arcachon bay, Rade de Brest and Thau lagoon).
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Larvae from Pacific oyster, Manila clam, European abalone and great scallop were subjected to two temperatures and two pH over the course of early development. RNAseq data was collected in order to evaluate which genes are modulated in response to stress.
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2bRAD genotyping will be used to estimate genetic diversity and connectivity among populations of Sabellaria alveolata. We will relate population genetic parameters with reef state characteristics.
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Whole genome pooled sequencing of individuals from 4 populations and 3 different color phenotype in order to uncover the genetic variants linked to color expression in the pearl oyster P. margaritifera.
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ddRAD genotyping was used to evaluate population connectivity and putative loci under selection in honeycomb worm from 13 sites spanning its distribution in the Atlantic and Mediterranean coasts.