WGS of SARS-CoV-2 by Oxford Nanopore Technology from raw wastewater samples collected in France, 2020-2021

Genetic diversity is often considered a key factor in a species’ ability to adapt to environmental changes and our aim was to understand the biological, ecological, and biogeographic factors that explain differences in genetic diversity among marine fish. Therefore, whole genomes of 160 individual marine fish were sequenced to a minimum depth of 15×, enabling near-complete representation of each diploid genome.

This dataset consists of metatranscriptomic sequencing reads corresponding to coastal micro-eukaryote communities sampled in Western Europe in 2018 and 2019.

scRNA-seq reads from a Pacific oyster (Crassostrea gigas) hemocyte preparation. Hemocytes were isolated from a unique immunologically naive animal (Ifremer Standardized Animal, 18 months) and single-cell drop-seq technology was applied to 3,000 individual hemocytes.

Comparison between 3 kits, Roche, Twist Biosciences and Illumina on the ability to enrich environmental samples to viral sequences. 5 sewage samples were extracted in triplicats with selective extraction protocol.

DNA sequencing of Crassostrea gigas Pacific oyster spat infected in the wild with OsHV-1 virus in 4 French oyster basins (Marennes Oleron Bay, Arcachon bay, Rade de Brest and Thau lagoon).

The eleven collected wild strains of T. lutea were compared phenotypically, in particular with regard to their pigment and lipid profiles. The genome of each T. lutea strain was also sequenced to investigate the genetic structure and genome organisation of this species. Collected data were summarized in a genome browser to provide easy-to-use support for the scientific community (https://genomes-catalog.ifremer.fr). This provides an important resource- to understand, exploit and predict the biodiversity of this species.

Develop parentage assignment panels using genetic fingerprinting of pearl oysters for use in commercial hatcheries and research to manage pedigrees in order to limit the risks of loss of genetic variability and increased inbreeding of commercial lines.

This study aims to compare different metabarcoding sequences of commercially fished shrimps collected by tree counties on the North Brazil Shelf Large Marine Ecosystem

Sequenced samples are city center wastewater sampled by passive samplers. Variants are identified by Illumina Miseq sequencing.